Custom PAb Production
Polyclonal antibodies (pAbs) are a type of antibody produced by injecting a specific antigen into lab animal. They are usually generated by different B cell clones in the body and can recognize and bind to many different epitopes of a single antigen. pAbs have a high affinity and specificity for the antigen, making them effective tools in research and diagnostics. They are commonly used in applications such as Western blotting (WB), ELISA, and immunohistochemistry (IHC) due to their ability to detect multiple epitopes on the same protein. Our comprehensive polyclonal antibody services include production of custom polyclonal antibodies in mouse, rat, rabbit, sheep, chicken, Guinea pig. BOT Bioscience provides one-stop solutions for your custom polyclonal antibody production, taking your project all the way from antigen design, peptide synthesis, and animal immunization, to antibody purification and QC with ELISA test.
Mouse Polyclonal Antibody Production Service
Mouse polyclonal antibodies are a mixture of immunoglobulins produced by multiple different B lymphocyte clones in immunized mice. When a mouse is immunized with a complex antigen, which typically contains multiple epitopes, various B cells recognize distinct epitopes on the antigen and produce a diverse set of antibodies. These antibodies collectively form polyclonal antibodies, each targeting different regions (epitopes) of the antigen. They offer high sensitivity and robustness for detecting antigens, even with minor variations. BOT Bioscience can provide customers with polyclonal antibody preparation services, including antigen production, antibody preparation, antibody purification, antibody labeling, antibody modification, etc. Customers can also choose the service plan suitable for themselves according to their own needs.
Rabbit polyclonal antibodies are a heterogeneous mixture of antibodies produced by multiple Bcell clones, recognizing different epitopes of the same antigen. They exhibit high affinity, sensitivity, and broad antigen detection, making them ideal for research applications like Western blotting, immunohistochemistry, and ELISA. Due to rabbits' strong immune responses, these antibodies often have better specificity and performance compared to those from other species. BOT Bioscience can provide customers with polyclonal antibody preparation services, including antigen production, antibody preparation, antibody purification, antibody labeling, antibody modification, etc. Customers can also choose the service plan suitable for themselves according to their own needs.
Rat polyclonal antibodies are a diverse mix of antibodies generated by multiple B-cell clones in rats, targeting various epitopes of the same antigen. They combine good specificity with strong immunogenicity, making them useful for applications like ELISA, flow cytometry, and neutralization assays. BOT Bioscience can provide customers with polyclonal antibody preparation services, including antigen production, antibody preparation, antibody purification, antibody labeling, antibody modification, etc. Customers can also choose the service plan suitable for themselves according to their own needs.
Sheep are used for polyclonal projects requiring large volumes and often used as the host species for the generation of secondary antibodies. These more traditional animal models respond well to a broad range of antigens and have a high affinity and sensitivity. The resulting antibodies are particularly adapted to small antigens or small epitopes. BOT Bioscience can provide customers with polyclonal antibody preparation services, including antigen production, antibody preparation, antibody purification, antibody labeling, antibody modification, etc. Customers can also choose the service plan suitable for themselves according to their own needs.
Chicken antibodies have a very high yield. Eggs are used to isolate all antibodies. Over time, a large number of antibodies can be generated since thel amount of antibodies from 10 eggs is equivalent to the amount of antibodies from one rabbit. Thus, chicken antibodies are an excellent substitute forl sheep and goat antibodies, especially when a significant quantity of antibodies is required. Compared with mammalian IgG antibodies, chicken IgY has higher specificity, affinity, and a lower risk of cross-reactivity. BOT Bioscience can provide customers with polyclonal antibody preparation services, including antigen production, antibody preparation, antibody purification, antibody labeling, antibody modification, etc. Customers can also choose the service plan suitable for themselves according to their own needs.
Guinea Pig Polyclonal Antibody Production Services
Guinea pig polyclonal antibodies offer unique advantages in cross-species research due to the evolutionary distance between guinea pigs and common laboratory animals like mice, rats, and rabbits. This often reduces background reactivity in dual-species experimental setups, making them particularly useful for double-labeling studies or when minimizing cross-reactivity with endogenous antibodies in host species is critical. BOT Bioscience can provide customers with polyclonal antibody preparation services, including antigen production, antibody preparation, antibody purification, antibody labeling, antibody modification, etc. Customers can also choose the service plan suitable for themselves according to their own needs.
